BMT LAB UNIT, NCI,
Cairo University
7th
Flow Cytometry Workshop 11th –15th October 2008
Program
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Registration
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Saturday 11th October |
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8:15
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Registration
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8:45
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Welcome
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9:00
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Lecture 1
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Introduction to Flow Cytometry
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Michael Ormerod
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10:45
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Lecture 2
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Immunofluorescence and colour compensation
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Michael Ormerod
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11:30
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Coffee break
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12:00
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Lecture 3
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Sample preparation and staining, data acquisition
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Vera Donnenberg
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12:45
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Lecture 4
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Instrumental set up: calibration and compensation
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Vera Donnenberg
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13:30
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Lunch
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14:30
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Practical
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Group A (Lab)
Introduction to the Flow Cytometer & Colour compensation
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Michael Ormerod
Vera Donnenberg
Khaled Shaaban
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Group B (Conference room)
Data Introduction and Gating
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Nahla El Sharkawy
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16:15
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Group A (Conference room)
Data Introduction and Gating
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Khaled Shaaban
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Group B (Lab)
Introduction to the Flow Cytometer & Colour compensation
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Michael Ormerod
Vera Donnenberg
Nahla El Sharkawy
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Sunday 12th October
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9:00
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lecture 1
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Data analysis, including gating
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Michael Ormerod
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10:00
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lecture 2
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Immunophenotyping of leukemia and lymphoma
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Paul Wallace
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11:00
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Coffee break
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11:30
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lecture 3
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Principles of rare event detection
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Vera Donnenberg
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12:30
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lecture 4
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Multi-drug resistance
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Vera Donnenberg
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13:30
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Lunch
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14:45 –18:00
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Practical
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Group A(Lab) Tracking Immune cell function
& Proliferation
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Paul Wallace
Nahla El-Sharkawy
Azza Kamel,
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14:45 –18:00
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Group B (Conference room)
Case Presentation including some cases for MRD & MDR
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Khaled Shaaban
Azza Kamel,
Vera Donnenberg
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Monday 13th October
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9:15
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lecture 1
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Designing flow cytometry experiment
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Albert Donnenberg
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10:15
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lecture 2
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Spectral compensation
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Albert Donnenberg
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11:15
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Coffee break
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11:45
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lecture 3
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Image Stream
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Paul Wallace
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12:45
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lecture 4
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Imaging flow cytometry
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Albert Donnenberg
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13:45
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Lunch
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14:45 –18:00
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Workshop
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Group B(Lab): Tracking Immune cell function
& Proliferation
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Paul Wallace
Khaled Shaaban
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14:45 –18:00
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Group A(Conference room): Case Presentation
including some cases for MRD & MDR
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Nahla El Sharkawy
Azza Kamel,
Vera Donnenberg
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Tuesday 14th October |
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9:15
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lecture 1
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Identification of antigen specific T cells
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Paul Wallace
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10:15
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lecture 2
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Searching for metastatic tumor in histologically
negative bone marrow aspirates
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Vera Donnenberg
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10:45
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Coffee break
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11:45
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lecture 3
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DNA Analysis and the cell cycle
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Michael Ormerod
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12:45
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lecture 4
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Combining DNA analysis and immunoflorescence
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Michael Ormerod
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13:45
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Lunch
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15:00
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Practical
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Group A (Lab)
Practical for DNA staining
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Michael Ormerod
Paul Wallace
Nahla El Sharkawy
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Group B (Conference room)
DNA analysis and presentations
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Khaled Shaaban
Azza Kamel
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16:30
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Group B (Lab)
Practical for DNA staining
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Michael Ormerod
Paul Wallace
Khaled Shaaban,
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Group A (Conference room)
DNA analysis and presentations
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Nahla El Sharkawy
Azza Kamel
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18:00
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Close
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Wednesday 15th October
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9:15
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Lecture 1
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Luminex: general technique
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Ronald Vos & Francois Topin
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10:15
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Lecture 2
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Luminex Assays
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Ronald Vos & Francois Topin
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11:15
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Coffee break
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11:45
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lecture 3
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Application of HLA on Luminex
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Paul Wong
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12:45
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Lecture 4
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XMP technology applications
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Paul Wong
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13:45
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Lunch
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14:45
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Workshop
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Group A (Lab)
XMP technology applications
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16:15
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Group B (Lab)
XMP technology applications
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17:45
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Round Table Discussion, Certificates ceremony
& close of workshop
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18:15
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Close
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