The First International Workshop at 

Cairo University Research Park

Application of Genetic Engineering in Biotechnology

Genetic engineering techniques have been applied in numerous fields including research, agriculture, industrial biotechnology, and medicine. Enzymes used in laundry detergent and medicines such as insulin and human growth hormone are now manufactured in GM cells, experimental GM cell lines and GM animals such as mice are being used for research purposes, and genetically modified crops have been commercialized.

It is a pleasure to announce that Cairo University Research Park (CURP) will launch a Genetic Engineering Workshop to attract applicants with highest intellectual and scientific skills in the area of biology. This workshop combines the opportunities to perform the latest, as well as commonly used molecular biology techniques for cloning of genes, sequencing, and sequence analysis with gene expression particular attention to the principles underlying each technique.

This workshop will be held from Aug 17^th to Sep 5^th, 2013 and carried out by the Molecular Genetics Group at Cairo University Research Park.

This workshop's hands-on activities were including: 

·        Isolation of total RNA and purify the mRNA.

·        Construction of cDNA library.

·        Gene cloning:  specific gene and cDNA library construction.

·        Cloning of PCR product in bacterial plasmid.

·        Transformation in bacterial cells.

·        Colony pick up and colony PCR.

·        DNA plasmid extraction.

·        Digestion DNA with restriction enzymes.

·        DNA gel electrophoresis and data analysis.

·        Isolation of novel tissue-specific genes from cDNA libraries.

·        DNA sequencing & DNA fingerprinting.

·        Gene expression analysis using microarray.

·        Single-cell analysis of gene expression by fluorescence microscopy.

·        Genomics and bioinformatics.

·        Protein expression analysis (Western Blotting and ELISA).

No previous experience in molecular biology is required or expected. Twenty participants will be selected from a variety of disciplines and academic backgrounds, including principal investigators, directors of programs, medical doctors, postdoctoral fellows, graduate students, research assistants, sales associates, equipment engineers, etc.

The three-week long courses cover in-depth DNA cloning, PCR, DNA sequencing, genomics and bioinformatics and gene expression. Learn hands-on techniques used in gene expression analysis including, quantitative RT-PCR, bioinformatics and genomics and protein expression.

Registration deadline is Thursday, July 20^th 2013.

Enrollment is limited to 20 participants.

Registration is open to all Nile River and Middle East Countries. Notification of acceptance will be emailed on July 22^nd, 2013. Please register only if you are able to attend the entire workshop. Registrants must mail in a reservation check for $100 to guarantee space in the workshop. It will be held and returned at the workshop, but will be forfeited for no-shows. Mail checks to: Faculty of Agriculture Bank Account. You will not be considered for attendance if we do not receive your check. 

Organizer:

Haggag S. Zein, PhD

Associate Professor Genetics Department

Directory of Biotechnology Labs

Cairo University Research Park

Faculty of Agriculture Cairo University

Tel/Fax: + 20235699182

E-mail:

farpbiotech@gmail.com

Website: http://www.farp.cu.edu.eg

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